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Tn5 DNA Library for 1 ng DNA br Acknowledgement We acknowled
2020-08-04
Acknowledgement We acknowledge for funding support from Maryada Foundation, a DoD/Army Contract No. W911NF-06-1-0095, and National Institutes of Health through the New England Center of Excellence for Biodefense (Grant AI057159-01). Introduction The Gram-negative bacterium Lysobacter sp. XL1
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All eukaryotes possess several E s
2020-08-04
All eukaryotes possess several E2s ranging from about 8 to 11 in certain apicomplexans and Giardia to a little over 50 in certain multicellular plants and animals (Supplementary material). Most of them, barring those in the autophagy pathway (Apg3 and Apg10), are relatively close to each other in s
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In summary we carried out the ligation
2020-08-04
In summary, we carried out the ligation of DNA fragments with cohesive ends using T4 vinorelbine receptor immobilized on ferromagnetic particles and found that the ligation efficiency was increased under a radio frequency alternating magnetic field caused by heat generation from the particles. In t
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br Introduction Gas hydrates are crystalline solid compounds
2020-08-03
Introduction Gas hydrates are crystalline solid compounds in which gas molecules (called guest molecules) are surrounded by water molecules, forming a regular cavity crystalline structure. Gas molecules with a desirable shape and size such as methane, ethane, propane, carbon dioxide and etc. can
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According to these results the power
2020-08-03
According to these results, the power GaN seems more robust than existing technologies (VDMOS, Superjunction, IGBT,) [5,10] and [11]. Comparing with the SiC technology, the previous works show a low SEE tolerance [[12], [13], [14]] and [15]. The SOA of power GaN devices can be equivalent to the IGBT
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Over the last years it has
2020-08-03
Over the last 5 years, it has become clear that proper regulation of apoptosis is dependent on appropriate ubiquitylation of various cellular components (Vucic, Dixit, & Wertz, 2011). Here, we have focused on IAP proteins that contain a RING domain, but other E3 ligases such as MDM2, which is the pr
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Metakaolin is the product of calcination of
2020-08-03
Metakaolin is the product of calcination of kaolinite rich clay at temperatures between 600 and 850 °C [11], [12] depending on the purity and the crystallinity of the initial clay. The geopolymers from metakaolin as aluminosilicate source have shown promising results in previous works [13], [14], [1
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As a co factor CIITA lacks the ability to
2020-08-03
As a co-factor, CIITA lacks the ability to recognize and directly bind to specific DNA elements to regulate transcription; instead, it relies on interaction with sequence-specific transcription factors (TFs) to be recruited to the chromatin. Therefore, the observation that CIITA binds to a specific
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br Estrogens and the brain Interestingly some of the
2020-07-31
Estrogens and the brain Interestingly some of the pathways of steroid actions in peripheral tissues are the same as in equol while others are different. One of the first descriptions of a steroidal effect in the brain was their sedative and analgetic effect [12], [13], [14], [15]. According to th
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As mentioned above EBI and
2020-07-31
As mentioned above, EBI2 and its ligand show some similarities to the sphingosine 1-phosphate (S1P)/S1P-receptor system, which mediates T cell egress from lymph nodes. Blockade of this system yielded in the first oral drug for MS patients named fingolimod. Therefore, the hope that EBI2 deficiency wo
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What are the molecular signals that
2020-07-31
What are the molecular signals that guide Tfh cells as they navigate around the lymph node in the course of the immune response? It was recently reported that Tfh cells inside GCs have high expression of SIPR2, which acts to repel them from the S1P-rich lymph in the SCS and promote their retention i
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br Materials and methods Transformation was
2020-07-31
Materials and methods Transformation was performed by electroporation [24]. The EZ-Tn5™ transposon mutants in A. baumannii ATCC 17978 were generated by using the EZ-Tn5™ insertion kit (Epicentre Biotechnologies) as previously described [11]. Surface-associated motility. Motility plates were c
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br Materials and methods Transformation was
2020-07-31
Materials and methods Transformation was performed by electroporation [24]. The EZ-Tn5™ transposon mutants in A. baumannii ATCC 17978 were generated by using the EZ-Tn5™ insertion kit (Epicentre Biotechnologies) as previously described [11]. Surface-associated motility. Motility plates were c
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We also checked if the DGKs are directly involved
2020-07-31
We also checked if the DGKs are directly involved in the PA formation process in response to EBL treatment. This could point at many PA-binding proteins and regulation of PA-dependent processes that are involved in maintaining of cell respiration and BRs signaling. dgk3, dgk1dgk2, dgk5dgk6 and dgk1
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Several antimalarial drugs in clinical studies target pyrimi
2020-07-30
Several antimalarial drugs in clinical studies target pyrimidine nucleotide biosynthesis , because parasites rely on fast and large replication of DNA to infect during liver and blood stages. Dihydroorotate dehydrogenase (DHODH) catalyzes the oxidation of -dihydroorotate (L-DHO) to -orotate in the
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